enzymatic detoxification of don in transgenic plants via expression of fusarium graminearum tri101 gene

نویسندگان

akram ahmadizadeh

forough sanjarian

kamahldin haghbeen

چکیده

fusarium graminearum is causal agent of economically catastrophic disease of cereal fusarium head blight (fhb) around the world. in addition to causing a loss of yield, this fungus causes serious threats to humans and animals due to the contamination of grain with the trichothecene mycotoxin. tri101 gene, a fusarium spp. gene, encodes an enzyme that transfers an acetyl group to the c3 hydroxyl of trichothecenes. we introduced tri101 gene from f. graminearum to tobacco to test its usefulness for decontamination of mycotoxins. the acetyltransferase activity of this gene was detected in transgenic plants. the growth pattern of t1 seedling was examined at present of deoxynivalenol (don) and crude extract of mycotoxin. a significant difference in growth rate was seen in plants expressing tri101 as compared to wild type in don assay. there was no significant difference in growth rate of the wild type and transgenic plants in presence of a mixture of mycotoxins. according to the results, it seems although fgtri101 enzyme is suitable for detoxification of don, more studies are required to evaluate its performance in detoxification of mixture of fusarium toxins.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Enzymatic detoxification of Don in transgenic plants via expression of Fusarium graminearum Tri101 gene

Fusarium graminearum is causal agent of economically catastrophic disease of cereal Fusarium Head Blight (FHB) around the world. In addition to causing a loss of yield, this fungus causes serious threats to humans and animals due to the contamination of grain with the trichothecene mycotoxin. TRI101 gene, a Fusarium spp. gene, encodes an enzyme that transfers an acetyl group to the C3 hydroxyl ...

متن کامل

Expression of 3-OH trichothecene acetyltransferase in barley (Hordeum vulgare L.) and effects on deoxynivalenol

Fusarium head blight (FHB), caused primarily by Fusarium graminearum, has been the most destructive disease of barley (Hordeum vulgare L.) in the USA since the early 1990s, resulting in large economic losses for growers. The fungus produces the mycotoxin deoxynivalenol (DON), a protein synthesis inhibitor, which is harmful to humans and livestock. Chemically modifying DON could reduce DON accum...

متن کامل

Expression Analysis of PKS13, FG08079.1 and PKS10 Genes in Fusarium graminearum and Fusarium culmorum

Background: Identification and quantification of mycotoxins produced by Fusarium species are important in controlling fungal diseases. Objectives: Potential of zearalenone, butenolide and fusarin C production was investigated in five Fusarium graminearum and five F. culmorum isolates at molecular level. Materials and Methods: Presence of PKS13, FG08079.1 and PKS10 genes, associated with produ...

متن کامل

Transgenic Wheat Expressing a Barley UDP-Glucosyltransferase Detoxifies Deoxynivalenol and Provides High Levels of Resistance to Fusarium graminearum.

Fusarium head blight (FHB), mainly caused by Fusarium graminearum, is a devastating disease of wheat that results in economic losses worldwide. During infection, F. graminearum produces trichothecene mycotoxins, including deoxynivalenol (DON), that increase fungal virulence and reduce grain quality. Transgenic wheat expressing a barley UDP-glucosyltransferase (HvUGT13248) were developed and eva...

متن کامل

Comparison of Trichothecene Biosynthetic Gene Expression between Fusarium graminearum and Fusarium asiaticum

Nivalenol (NIV) and deoxynivalenol (DON) are predominant Fusarium-producing mycotoxins found in grains, which are mainly produced by Fusarium asiaticum and F. graminearum. NIV is found in most of cereals grown in Korea, but the genetic basis for NIV production by F. asiaticum has not been extensively explored. In this study, 12 genes belonging to the trichothecene biosynthetic gene cluster were...

متن کامل

Expression in cereal plants of genes that inactivate Fusarium mycotoxins.

Trichothecene 3-O-acetyltransferase (encoded by Tri101) inactivates the virulence factor of the cereal pathogen Fusarium graminearum. Zearalenone hydrolase (encoded by zhd101) detoxifies the oestrogenic mycotoxin produced by the same pathogen. These genes were introduced into a model monocotyledon rice plant to evaluate their usefulness for decontamination of mycotoxins. The strong and constitu...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
progress in biological sciences

ناشر: university of tehran

ISSN 1016-1058

دوره 3

شماره 1 2013

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023